General Reagents

Unless stated otherwise, all reagents were obtained from Becton Dickinson Microbiology Systems (Sparks, MD), Bio-Rad Laboratories (Hercules, CA), Boehringer Manheim Corporation (Indianapolis, IN), Fisher Scientific Company (Fair Lawn, NJ), Gibco BRL Life Technologies (Paisley, Scotland), J.T. Baker (Phillipsburg, NJ), Pharmco Products (Brookfield, CT), Pierce (Rockford, IL), or Sigma Chemical Company (St. Louis, MO). Liquid sterilization was achieved by autoclaving at 120ºC and 25psi for 30 minutes, or by microfiltration (0.2 mm, Pall Corporation, Ann Arbor, MI).

Bacteria and Cells

CF31 human dermal fibroblasts isolated from a 31 year-old Caucasian female (BioWhittaker, Walkersville, MD)

XL1-blue E.coli competent bacteria (Stratagen, Austin, TX)

Abstract

Introduction

Wound Healing

Chronic Ulcer Theory

Current Treatments

Fibronectin

Hyaluronan

Product Design

Product Design

Materials

Materials

Methods

Recombinant FN Fragment Purification

Cell Culture

Agarose Droplet Migration Assay

Preparation of Hyaluronan Gel

Hyaluronan Gel Three Dimensional Migration Assay

Hyaluronan Gel Cell Toxicity Tests

Agarose-Droplet Migration Assay Validation

Agarose-Droplet Migration Assay Validation

Agarose-Droplet Migration Assay Results

Agarose-Droplet Migration Assay Results

Hyaluronan Migration Assay Validation

Hyaluronan Migration Assay Validation

Hyaluronan Migration Assay Results

Hyaluronan Migration Assay Results

Discussion and Conclusion

Discussion and Conclusion

Bibliography

Bibliography

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